Lysis buffer

Reagent	Final concentration	Amount
Urea	8 M	4.8 mg
Water	N/A	6.748 mL
TEAB, pH 8 (1 M)	200 mM	2 mL
Nicotinamide (30 mM)	3 mM	1 mL
Sodium chloride (5 M)	75 mM	150 μL
HALT protease/phosphatase single-use inhibitor cocktail (100x)	1x	100 μL
Trichostatin A (5 mM)	1 μM	2 μL
Total	N/A	10 mL

Note on storage conditions: The lysis buffer should be prepared immediately before use.