8 M urea, 50 mM TEAB, pH 8 buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| Urea | 8 M | 4.8 g |
| Water | N/A | 9.5 mL |
| TEAB, pH 8 (1 M) | 50 mM | 500 μL |
| Total | N/A | 10 mL |
Note on storage conditions: The 8 M urea solution should be prepared immediately before use.
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