TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| X. oryzae pv. oryzae | ||
| BXO1 | Natural isolate (Chinsuria, West Bengal, India) | Lab collection |
| BXO112 | rif-1; Rifr derived from BXO1 | 29 |
| BXO113 | spv-2 rif-1 | 29 |
| BXO114 | spv-3 rif-1 | 29 |
| BXO115 | spv-4 rif-1 | 29 |
| BXO117 | spv-5 rif-1 | 29 |
| BXO119 | spv-6 rif-1 | 29 |
| BXO121 | spv-7 rif-1 | 29 |
| BXO123 | spv-8 rif-1 | 29 |
| BXO125 | spv-9 rif-1 | 29 |
| BXO127 | spv-10 rif-1 | 29 |
| BXO148 | rif-1; Eps+ Vir+ recombinant of BXO125 | This study |
| BXO149 | rif-1; Eps+ Vir+ recombinant of BXO125 | This study |
| BXO150 | rif-1; Eps+ Vir+ recombinant of BXO125 | This study |
| BXO151 | BXO125/pSD2 | This study |
| BXO152 | BXO125/pSD1 | This study |
| E. coli | ||
| DH5α | F′ end A1 hsdR17 (rK− mK+) supE44 thi-1 recA1 gyrA relA1 φ80dlacZ ΔM15 Δ(lacZYA-argF)U169 | Lab collection |
| S17-1 | RP4-2-TC::Mu-Km::Tn7 pro hsdR recA | 33 |
| Plasmids | ||
| pSD1 | pUFR034 + 36 kb of BXO1 DNA containing the gum gene cluster | 5 |
| pSD2 | pUFR034 + 14.2 kb of BXO1 DNA containing part of the gum gene cluster | 5 |
| pRR7 | pBKS + the 4.5-kb BamHI fragment from pSD2 | This study |