TABLE 1.
Bacterial strains and plasmids used
| Strain or plasmid | Genotype | Reference |
|---|---|---|
| E. coli strain | ||
| JA300 | F−thr leuB6 trpC1117 thi rpsL20 hsdS | 1 |
| JA300A | Same as JA300, but with ΔacrAB::cat | This study |
| JA300T | Same as JA300, but with ΔtolC | 4 |
| JA300TA | Same as JA300, but with ΔtolC ΔacrAB::cat | This study |
| JA300E | Same as JA300, but with emrB::kan | This study |
| JA300AE | Same as JA300, but with ΔacrAB::cat emrB::kan | This study |
| OLS111 | emrB::kan rpoS::tet | A. Xiong and A. Matin |
| Plasmid | ||
| pLTolC | 2.5-kb EcoRI-HindIII fragment containing the tolC gene in pMW119 oriented in the same direction as Plac | 4 |
| pLKAcrAB | 4.4-kb AvaI-EcoT22I fragment containing the acrAB genes in pMW219 oriented in the same direction as Plac | This study |
| pLKEmrAB | 3.2-kb PCR product containing the emrAB genes in pMW219 oriented in the same direction as Plac | This study |
| pLKYhiUV | 4.6-kb PCR product containing the emrAB genes in pMW218 oriented in the same direction as Plac | This study |