Table 2.
Antibodies used in the western blotting analysis and their properties.
| Antibody | Specificity | Type | Dilution for WB | Source | Catalog number |
|---|---|---|---|---|---|
| ACTB/β-Actin | ACTB/β-Actin | Poly- | 1:5000 | SAB | 21338 |
| 4G8 | β-Amyloid, 17–24 | Mono- | 1:200 for IF | Biolegend | 800708 |
| Aβ | β-Amyloid | Mono- | 1:1000 for WB | CST | 8243 |
| APP | APP | Mono- | 1:1000 for WB | Biolegend | 800901 |
| p-APP | p-APP | Mono- | 1:1000 for WB | CST | 6986 |
| PSEN1 | PSEN1 | Poly- | 1:1000 for WB | SAB | 24526 |
| BACE1 | BACE1 | Poly- | 1:1000 for WB | SAB | 54901 |
| IRF1 | IRF1 | Mono- | 1:1000 for WB | CST | 8478 |
| LC3 | Total LC3B | Poly- | 1:1000 for WB 1:200 for IF |
CST | 2775 |
| ATG5 | Total ATG5 | Mono- | 1:1000 for WB | CST | 12994 |
| ATG7 | Total ATG7 | Mono- | 1:1000 for WB | CST | 8558 |
| SQSTM1/p62 | Total SQSTM1/p62 protein | Mono- | 1:1000 for WB | CST | 23214 |
| VMA21 | VMA21 vacuolar-type H+-ATPase homolog | Mono- | 1:500 for WB | Proteintech | 21921 |
| ADRB2 | adrenergic receptor, beta 2 | Mono- | 1:1000 for WB | Abcam | 182136 |
| MTOR | Total MTOR | Mono- | 1:1000 for WB | CST | 2983 |
| p-MTOR | Phospho-MTOR (Ser2448) | Mono- | 1:1000 for WB | CST | 5536 |
| PRKAA/AMPK | PRKAA1/AMPK alpha 1 | Mono- | 1:1000 for WB | SAB | 48827 |
| p-PRKAA/AMPK | Phospho-PRKAA/AMPKα (Thr172) | Mono- | 1:1000 for WB | CST | 2535 |
Mono-, monoclonal; poly-, polyclonal.