Figure 5.

Transplantation of fat-1 microbiome decreases weight gain, enhances metabolic parameters and alleviates intestinal alteration in mice fed a HFD.

WT mice were transplanted with the microbiome of WT or fat-1 mice and fed CTL or HF diet for 12 weeks. a: Weight gain curves (n = 8 per group). b: OGTT and (inset) mean area under the curve (AUC) measured between 0 and 120 min after glucose loading (n = 6 per group). c: ITT measured between 0 and 90 min after glucose loading (n = 6 per group). d: Food intake has been carried out at 6 and 8 weeks of the feeding period (n = 8 per group). e: Intestinal permeability assay: Plasma FITC-dextran 4 (DX, 4,000 molecular weight) oral challenge was measured in mice (n = 8 per group) fed CTL or HF diet for 12 weeks. f: Direct plasma quantitation of 3-β-hydroxymyristic (OH) acid concentration by gas chromatography-mass spectrometry in mice fed CTL or HF diet for 12 weeks (n = 8 per group). Data are shown as mean ± SEM, and differences were analyzed by Tukey’s multiple comparison test. Data with different superscript letters (a, b, c) are significantly different at p < 0.05. WTW CTL: WT mice transplanted with microbiota of WT mice fed a CTL diet, WTW HFD: WT mice transplanted with microbiota of WT mice fed a HFD, WTF CTL: WT mice transplanted with microbiota of fat-1 mice fed a CTL diet, and WTF HFD: WT mice transplanted with microbiota of fat-1 mice fed a HFD. OGTT: Oral Glucose Tolerance Test. ITT: Insulin Tolerance Test.