Figure 6.

Transplantation of the fat-1 microbiome prevents the increase of the thickness of the colonic mucus layer in mice fed the HF diet.

WT mice were transplanted with the microbiome of WT or fat-1 mice and fed CTL or HF diet for 12 weeks. a: Representative photographs of Carnoy-fixed colonic tissue sections stained with Alcian blue/periodic acid-Schiff of mice transplanted with the microbiome of WT or fat-1 mice and fed a CTL or HF diet (n = 8 per group) used for mucus layer thickness measurements (scale bars, 100 µm). b: Colonic mRNA expression of Muc2 and Klf4 (n = 8 per group). Data are shown as mean ± SEM, and differences were analyzed by Tukey’s multiple comparison test. Bars assigned different superscript letters (a, b, c) are statistically different at p < 0.05. WTW CTL: WT mice transplanted with microbiota of WT mice fed a CTL diet, WTW HFD: WT mice transplanted with microbiota of WT mice fed a HFD, WTF CTL: WT mice transplanted with microbiota of fat-1 mice fed a CTL diet, and WTF HFD: WT mice transplanted with microbiota of fat-1 mice fed a HFD. MUC2: mucin 2. KLF4: Krüppel-like factor 4.