Figure 7.

Transplanting fat-1 microbiome protects HFD-fed WT mice against colonic endoplasmic reticulum stress.

WT mice were transplanted with the microbiome of WT or fat-1 mice and fed CTL or HF diet for 12 weeks. a: Colonic mRNA expressions of Chop, Atf4, and Edem1 I (n = 8 per group). b: Colonic mRNA expression of Lc3, Ulk1, P62 and Vamp8 (n = 8 per group). Data are shown as mean ± SEM, and differences were analyzed by Tukey’s multiple comparison test. Bars assigned different superscript letters (a, b, c) are statistically different at p < 0.05. WT mice transplanted with microbiota of WT mice fed a CTL diet, WTW HFD: WT mice transplanted with microbiota of WT mice fed a HFD, WTF CTL: WT mice transplanted with microbiota of fat-1 mice fed a CTL diet, and WTF HFD: WT mice transplanted with microbiota of fat-1 mice fed a HFD. CHOP: C/EBP Homologous Protein. ATF4: Activating transcription factor 4. Edem1: ER degradation enhancer, mannosidase alpha-like 1. LC3: Microtubule-associated protein 1A/1B-light chain 3. ULK1: Unc-51 like autophagy activating kinase. P62: Sequestosome-1. VAMP8: vesicle-associated membrane protein; 8.