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. Author manuscript; available in PMC: 2024 May 29.
Published in final edited form as: ACS Nano. 2015 Jul 31;9(8):8231–8238. doi: 10.1021/acsnano.5b02595

Figure 2.

Figure 2.

Confocal imaging and modeling of diffusion profiles in HepG2 spheroids after 20 min exposure to fluorescent penetrants. (a) and (b) are calculated diffusion profile of CNT-TGFβ1-FITC and RhB, which compare to (c) and (d), permeation profiles of CNT-TGFβ1-FITC and RhB at central focal plane of the spheroid; (e) and (f) are the 3D surface plots of (c) and (d); H-axis represents the fluorescent intensity; the penetration depth is defined at the peak of fluorescent intensity. CNTs and TGFβ1 have green fluorescence in (c), (g) and (h); HepG2 cells in spheroids were stained red (CMTPX) before spheroid formation for images (g) and (h). Concentration of (d) free RhB (Red) is 1.1 × 10−4 mg/mL, and concentration of (h) free TGFβ1 is 3 μg/mL.