FIG. 2.
Swarm assay of LMB216. Cells of the indicated genotype were inoculated onto PAB–0.4% agar plates and grown at 37°C overnight. LMB1, the wild-type strain, has swarmed from the point of inoculation, which indicates that this strain is motile. LMB241 (sigD::pLM5 flgM::mini-Tn10), the negative control strain, exhibits growth from the position of inoculation, but does not swarm. LMB214 (fla/che PAΔ) and LMB216 (fla/che PAΔ flgM::mini-Tn10) also do not swarm, indicating that they are nonmotile. This indicates that in LMB216, activation of ςD by the removal of FlgM does not restore motility.