Extended Data Fig. 7. Dependency of JAM2 interaction with TWA1 and TWA1 orthologues on temperature.
a, Temperature dependence of JAM2 binding to TWA1 and variants. Y2H growth assay for histidine autotrophy. The JAM2-JAM2 pair (JAM2) served as positive control. b, Histidine autotrophic yeast growth in the Y2H assay provided by the binding of JAM2 fusion with Gal4-BD to Gal4-AD fusions of JAM2 (JAM2), TWA1, and orthologues from A. lyrata, S. alba, and a TWA1 version in which the Arabidopsis HVR has been exchanged for the corresponding domain of A. lyrata (TWA1_AlHVR). a, b, The inoculum was 0.020 optical density (OD) at wavelength 600 nm in growth medium lacking leucine, tryptophan, and histidine (-L,-W,-H), and data are mean ± SD, n = 3 per data point).