Extended Data Fig. 7. Proliferative niche endothelial cells promote matrix remodelling of the micro-niche.

a-d, Gene set enrichment analyses of ranked upregulated genes. NES, normalized enrichment score, p values were computed by permutation. n = 6 mice per group. a, Gene sets enriched in unlabelled total EC from 4T1-injected lungs compared to unlabelled total EC from D2.0R-injected lungs. b, Gene sets enriched in labelled EC from 4T1-injected lungs compared to matched unlabelled total EC. c, Gene sets enriched in labelled EC from D2.0R-injected lungs compared to matched unlabelled total EC. d, Gene sets enriched in labelled EC from 4T1-injected lungs compared to labelled EC from D2.0R-injected lungs. e, Expression of proliferative niche EC markers genes in individual capillary EC from the combined scRNAseq dataset (full set of filtered marker genes for all conditions are displayed in Supplementary Table 8). f, Gene scores for marker genes in e for individual EC from the combined scRNAseq dataset split by experimental timepoint. Dotted line indicates threshold of EC with niche gene score > 0.5. g, Localisation of predicted tumor cell-interacting EC in the capillary EC dataset split by timepoint. h, Presence of predicted tumor cell-interacting EC in control non-tumorous and primary lung tumor EC in a publicly available dataset (13,854 cells). i, Localisation of predicted tumor cell-interacting EC and biosynthetic EC identified in Extended Data Fig. 4 in the combined capillary EC dataset. j, Violin plot of biosynthesis gene scores in endothelial cells identified as proliferative niche EC (niche score > 0.5, red colour) and non-niche EC (niche score < 0.5, grey colour).