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. 2024 May 29;14:12361. doi: 10.1038/s41598-024-56261-5

Table 1.

Main quality parameters of C. waltheriae and T. diversipes assemblies.

Metric C. waltheriae T. diversipes
Total transcripts assembled 87,358 17,866a
40,796b
Number of clustered SuperTranscriptsc 18,208 11,998
SuperTranscripts ≥ 1000 nt 10,195 8051
p fragments mappedd 0.93176 0.86625
p bases uncoverede 0.0328 0.05047
Transrate optimal scoref 0.5904 0.50711
Complete BUSCOs (%) 93.4 88.5
Missing BUSCOs (%) 4.1 8.2

aNumber of transcripts obtained after the reference-based assembly.

bNumber of transcripts obtained after the reference guided de novo assembly.

cAfter removing contaminating sequences.

dProportion of reads pairs that mapped successfully.

eProportion of bases that were not covered by any read.

fThis discrete score represents the accuracy and completeness of the assembly based on the assessment of alignments between contigs and reads. The minimum score is 0, while the maximum is 1.0. For a description of the components used in the calculation of this metric, see Smith-Unna et al.38.