Fig. 4. ELISA analysis of endogenous A. muciniphila neuraminidases with human mucin TR reporters.

a Immobilized recombinant purified mucin reporters (250 ng/mL) were incubated with recombinant A. muciniphila Amuc_0625 neuraminidase (1.4 mU) or control C. perfringens neuraminidase (2.5 mU) and binding of pasteurized A. muciniphila (5 ×10⁸ CFU/mL) tested. b Analysis as in a) with the sialic acid binding pan-specific Lectenz (2.0 μg/mL). Plates were read at 450 nm. Bars represent the mean ± SD of 2 biological replicates. Source data are provided as a Source Data file.