FIGURE 1.

Schematics of long-read sequencing approaches: nanopore sequencing (Oxford Nanopore Technologies) and SMRT sequencing (Pacific BioSciences). (A) NS relies on the passage of the ssDNA through the membrane driven by the motor protein. The nucleotide bases are identified using the directed ionic current intensities arising from the motion of particles through the membrane. (B) During SMRT sequencing, dsDNAs are circularized into a SMRT bell. The SMRT bell contains the four fluorescently labeled nucleotides with unique emission spectra. The bases are distinguished by the alterations in the light emission spectra.