TABLE 1.
DNA oligonucleotides used in this study
| Primer | Sequence (5′→3′)a |
|---|---|
| YC42F | AACSTGCCTKCTTATYTCCTA |
| YC42R | CGGAACACTCAACATACTTKA |
| 5′42WD | CAGTCGACACCGCTACCCATCT |
| 3′42WD | ACGATATCCCTGACACGATCCATT |
| 5′42 | ACGTCCGCTATTCATCTTCTAT |
| 5′42Kpn | ATGGTACCTGCGGGTATGACAAAGGTC |
| 3′42Hind | TAAAGCTTACTTCGCCAGCATCGTTTGA |
| 3′42 | CTCGTTTGGGTCAGACTTTCTTG |
| 42-14V | GCCGACCGCGACGTCACCG |
| N-19N | CGGTCGGCAAGAACTGCTTGCTGATATCGTACACCACC |
| 42-120 | CTCAGACCGCTCTACGTGAC |
| 42-186I7N | CCACCAAAGAAGAATATTAAGAAATGCACG |
| 42Bgl | AAAGATCTACGGAATGGTTGTCGCAACG |
| 42Xba | AATCTAGACCGAGTCTCATCAAAGAATCGT |
a
Sequences for restriction endonuclease recognition sites are in italics. For mutagenic primers, sequences changed from the original DNA sequence of W. dermatitidis are underlined.