TABLE 2.
Change in the percentage of cell types induced by transformation and expression of wdcdc42 mutant allelesa
| Strainb | % of cells in form of:
|
||
|---|---|---|---|
| Yeast | Sclerotic bodiesc
|
||
| Phase I | Phase II | ||
| D42-60/303 | 99.3 | 0 | 0.6 |
| D42-60/Wt42 | 97.7 | 1.3 | 0.6 |
| D42-60/14V | 33.3 | 34.0 | 32.6 |
| D42-60/120A | 99.7 | 0 | 0.3 |
a
Values are the averages for two independent transformants. Three hundred yeast and sclerotic bodies were counted for each sample.
b
All transformants were derived from the wdcdc42 null strain Bd42-60. CDY was used as the basic growth medium, in which dextrose was replaced with soluble starch. Mid-log-phase cells harvested from a 25°C culture were diluted in a medium prewarmed at 37°C and incubated at this temperature for 3 days.
c
The term “sclerotic bodies” refers to an isotropically enlarged cell phenotype that contains multiple nuclei (phase I) and at least one transverse septum (phase II).