FIG. 1.

(a) Schematic representation of the rhaBAD and rhaT promoter regions. RNA polymerase and the two activator proteins CRP and RhaS are shown bound to DNA in their respective positions. (b) DNA sequences of the rhaBAD and rhaT promoter regions, extending from the −35 regions to the most upstream endpoint of the promoter fusions used in this work. The positions of the RhaS-binding sites are shown by everted arrows, and the positions of the CRP-binding sites are shown by inverted arrows. The −35 regions of each promoter are marked, and the upstream endpoints of promoter fusions with lacZ are identified by a “Δ.”