Fig. 2.

CircDCAF8 promotes proliferation, migration, invasion and EMT in HCC cells. A Relative expression of circDCAF8 in HCC cell lines and THLE-2 (human immortalized hepatocytes). B The knockdown efficiency of circCAF8 in Hep-G2 cells and overexpression efficiency in Hep-3B cells were determined by qRT-PCR. C, D EdU and colony formation assays evaluated the proliferation of sh-circDCAF8 cells and LV-circDCAF8 cells. Scale bar = 50 μm. E Wound healing assay determined cell migration ability. Scale bar = 100 μm. F Transwell assay measured invasion and migration ability with or without matrix. Scale bar = 500 μm. G, H Immunofluorescence and western blot detected the expression of EMT-related proteins. Scale bar = 50 μm. Data are representative of three independent experiments and are presented as means ± SDs. (*p < 0.05; **p < 0.01; ***p < 0.001)