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. 2024 May 30;22:517. doi: 10.1186/s12967-024-05233-4

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — CircDCAF8 acts as a sponge of miR-217. A Downstream miRNAs of circDCAF8 predicted by circbank and circinteractome databases. B Relative expression of downstream miRNAs was determined by qRT-PCR in circDCAF8 knockdown and overexpression cells. C Relative expression of miR-217 in human HCC tissues and paired adjacent nontumor tissues of 64 patients was determined by qRT-PCR. D Spearman correlation analysis showed circDCAF8 expression was negatively correlated with the miR-217 expression. E Pull down assays showed that miR-217 was enriched by the circDCAF8 probe. F RIP assay confirmed circDCAF8 and miR-217 could bind with RNA-induced silencing complex (RISC). G A schematic of wild-type (WT) and mutant (MUT) circDCAF8 luciferase reporter vectors. H Luciferase reporter assay unveiled the molecular combination of miR-217 with circDCAF8 wild type in HEK-293T cells. Data are presented as means ± SDs. (ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001)