Fig. 6.

NAP1L1 is a target gene of miR-217. A Downstream genes of miR-217 predicted by miRDB, Tarbase, Starbase and TargetScan databases. B The overexpression and knockdown efficiency of miR-217 were determined by qRT-PCR. C Relative expression of downstream genes was determined by qRT-PCR in miR-217 overexpression and knockdown cells. D Relative expression of NAP1L1 in human HCC tissues and paired adjacent nontumor tissues of 64 patients was determined by qRT-PCR. E, F Spearman correlation analysis showed NAP1L1 expression was negatively correlated with the miR-217 expression(E) and positively correlated with circDCAF8 expression(F). G Relative expression of NAP1L1 was determined by qRT-PCR in circDCAF8 knockdown and overexpression cells. H, I Western blot was performed to detect protein expression levels of NAP1L1 in miR-217 and circDCAF8 knockdown or overexpression cells. J Luciferase reporter assay unveiled the molecular combination of miR-217 with circDCAF8 wild type in HEK-293T cells. Data are presented as means ± SDs. (ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001)