Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 May 29;23:186. doi: 10.1186/s12933-024-02224-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 6 — Slc39a14 and Slc39a8 are involved in the altered iron metabolism observed in vascular calcification. Venous blood was collected and serum ferrous iron levels were determined using an iron assay kit (A). Serum calcium and alkaline phosphatase (ALP) concentrations were measured and normalised to protein levels (B and C). Relative RNA levels of Runx2, Slc39a14, Slc39a8, DMT1, and TFRC were analysed by qPCR and normalised; *P < 0.05, **P < 0.01 (D). Total aortic proteins were used to determine the expression of Slc39a14, Slc39a8, and TFRC by western blotting (*P < 0.05, **P < 0.01, E and F). The expression of Slc39a14, Slc39a8, and TFRC in aortic sections was examined by immunohistochemical (IHC) staining. Black arrow head indicates the medial layer of artery. Scale bar: 50 µm (G). The expression levels of Slc39a14, Slc39a8, and TFRC were determined through immunofluorescence staining. White arrow head indicates the medial layer of artery. Scale bar: 20 µm (H)