Fig. 3.

Model involving the Fe-S cluster of human mitoNEET in the repair of the cluster of the cytosolic aconitase after ROS exposure. Under physiological conditions, mitoNEET (PDB ID: 7P0O¹⁵) harbors a [2Fe-2S]⁺ cluster and aconitase ACO1 (PDB ID: 2B3Y¹³⁹) accommodates a [4Fe-4S] cluster essential for its activity. Upon ROS exposure, mitoNEET [2Fe-2S] cluster is oxidized and aconitase [4Fe-4S] cluster is disassembled revealing the regulatory function of its apo-form termed IRP1 (see Section “Fe-S clusters as Lewis acids: Aconitases and dihydroxyacid dehydratases”). After stress removal, mitoNEET transfers its clusters to IRP1 converting it back to aconitase.