Table 4.
Items | Treatment 1 | SEM | p-value 2 | |||||
---|---|---|---|---|---|---|---|---|
CON | T1 | T2 | T3 | Treat | Linear | Quadratic | ||
Ruminal pH | 6.56a | 6.55a | 6.59a | 6.38b | 0.007 | 0.04 | 0.025 | 0.007 |
NH3-N, mg/mL | 6.96b | 7.79b | 10.50a | 3.87c | 0.540 | <0.01 | 0.177 | <0.01 |
MCP, mg/mL | 153.54ab | 124.55b | 150.05ab | 174.74a | 7.75 | 0.148 | 0.205 | 0.093 |
Total VFA, mmol/L | 97.05 | 100.43 | 96.04 | 98.40 | 1.70 | 0.843 | 1.000 | 0.981 |
Acetate mmol/L | 48.07 | 49.65 | 50.10 | 47.67 | 0.92 | 0.777 | 0.906 | 0.580 |
Propionate mmol/L | 41.13 | 42.29 | 37.42 | 42.88 | 1.10 | 0.344 | 0.919 | 0.696 |
Butyrate mmol/L | 7.84 | 7.99 | 8.52 | 7.85 | 0.22 | 0.717 | 0.814 | 0.679 |
Acetate: Propionate ratio | 1.17b | 1.16b | 1.37a | 1.11b | 0.04 | 0.067 | 0.985 | 0.315 |
VFA, volatile fatty acid.
Different supplementary levels (CON, control group; T1, 0.1% NSPases/kg DMI; T2, 0.3% NSPases/kg DMI; T3, 0.5% NSPases/kg DMI).
Treat, Linear and Quadratic represent treat, linear and quadratic effects, respectively, of NSPase supplementation.
a–cValues within a row with different superscripts differ significantly at p < 0.05.