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Real-Time Polymerase Chain Reaction to evaluate mRNA transcripts. OSM- oncostatin M (panel A), OSMR-oncostatin M receptor (panel B), mechanistic Target of Rapamycin (mTOR; panel C), α-SMA- Ki67- cell proliferation marker (panel D), alpha-smooth muscle actin (panel E), and p27kip1 (panel F). Data are presented as the mean ± SEM. *p<0.05, **p<0.01, ***p<0.001,and ****p<0.0001.
