Fig. 1.

Na,K-ATPase subunit α is overexpressed in colon cancer. (A) ATP1A1 RNA overexpression is significantly associated with colon adenocarcinoma. Bean plot analyses of ATP1A1 expression in 596 adenocarcinomas and 373 healthy colon samples reveal an approximately threefold difference in expression. The width of the horizontal lines represents the number of samples at each value, while the pink and yellow lines indicate the average ATP1A1 expression in normal colon and adenocarcinoma, respectively. The triple black asterisk indicates a P-value of 2.33×10⁻²³ obtained via a two-tailed Student's t-test. (B) Quantification of the colocalization between Na,K-ATPase, and β-catenin in normal colon and in advanced stages of cancer from human array sections. (C-E″) Immunohistochemistry from B shows strong colocalization between Na,K-ATPase and β-catenin in advanced colon cancers (inset). Error bars denote SEM (n≥3) (**P<0.01). Scale bars: 10 μm. Human array sections were quantified in triplicate fields.