TABLE 2.
Alginate production in different A. vinelandii strains
| Strain | Genotype | Mean alginate concna (mg/mg of protein) ± SEM (%) | Mucoidyb |
|---|---|---|---|
| AEIV | Wild type | 0.9 ± 0.2 (100) | + |
| AEA8 | AEIV, mucA | 5.9 ± 0.4 (651) | +++ |
| AEA4 | AEIV, mucABCD | 4.0 ± 0.15 (444) | ++++ |
| AEC2 | AEIV, mucC | 2.4 ± 0.4 (266) | ++ |
| ATCC 9046 | Wild type, muc-1 | 4.8 ± 0.3 (100) | +++ |
| JRA8 | ATCC 9046, mucA | 6.7 ± 0.8 (139) | +++ |
| JRA4 | ATCC 9046, mucABCD | 8.9 ± 0.7 (185) | +++++ |
| MLC2 | ATCC 9046, mucC | 4.0 ± 0.3 (83) | +++ |
| MLC4 | ATCC 9046, mucCD | 5.1 ± 0.2 (106) | +++ |
a
Alginate was determined in cells grown for 48 h on liquid Burke's nitrogen-free salts supplemented with 2% sucrose as a carbon source.
b
+ to +++++, least to most mucoidy, respectively.