TABLE 3.
Determination of A. vinelandii AlgU activity in the E. coli background
| Strain | Genotype | β-Galactosidase (U)a at min:
|
||
|---|---|---|---|---|
| 0 | 30 | 60 | ||
| CAG16037 | rpoH p3::lacZ | 8.75 | 10.8 | 9.17 |
| CAG22216 | rpoE::CmrrpoH p3::lacZ | ND | ND | ND |
| CAG22216/pJMSAT1 | A. vinelandii algU+ mucA+ | 1.12 | 1.6 | 2.0 |
a
β-Galactosidase activity reflects the level of E. coli rpoH p3 promoter which is fully dependent on ςE activity and was determined in cultures after shift to 42°C for the indicated time in minutes. Cells were grown on M9 medium as described previously (29). The data presented are the averages of three independent experiments. ND, not detected.