TABLE 2.
Doubling times and relative yield of oxidase mutants grown in liquid media
| Strain | Relevant genotype | Doubling time (min)a
|
Relative yieldb
|
||||
|---|---|---|---|---|---|---|---|
| NSMP | NSMPG | MM | NSMP | NSMPG | MM | ||
| 1A1 | Wild type | 41 ± 0.7 | 33 ± 1.7 | 49 ± 3.1 | 1.0 | 1.0 | 1.0 |
| LUW34 | ΔcydABCD | 42 ± 0.9 | 34 ± 1.2 | 48 ± 1.1 | 0.97 | 0.95 | 1.0 |
| LUW46 | ΔqoxABCD | 57 ± 0.9 | 32 ± 0.1 | 65 ± 2.0 | 0.57 | 0.95 | 0.78 |
| LUW137 | ΔythAB | 41 ± 0.9 | 31 ± 1.3 | 50 ± 2.1 | 1.0 | 0.96 | 0.98 |
| LUW142 | ΔctaCD | 39 ± 1.8 | 29 ± 1.9 | 50 ± 1.4 | 1.0 | 0.97 | 1.0 |
| LUW148 | ΔqoxABCD ΔctaCD ΔythAB | 59 ± 2.3 | 32 ± 0.5 | 63 ± 5.8 | 0.48 | 0.76 | 0.53 |
| LUW196 | ΔcydABCD ΔctaCD ΔythAB | 42 ± 1.5 | 32 ± 1.9 | 44 ± 0.8 | 1.0 | 1.0 | 0.99 |
a
Doubling times were calculated for strains in the exponential growth phase, grown at 37°C in different media. Values are the mean of at least three experiments. The degree of spread is indicated as the standard error of the mean.
b
Relative yield is defined as the highest optical density in early stationary phase in terminal oxidase mutant strain cultures relative to that in the wild-type strain cultures. Values are the means of at least three experiments. The variation is less than 6%.