TABLE 3.
Relative frequency of transformants obtained in different B. subtilis strains transformed with plasmid pSPOX
| Strain | Relative frequency of transformants under culture conditionsa
|
|||
|---|---|---|---|---|
| +O2
|
−O2
|
|||
| +IPTG (+ cyt. aa3) | −IPTG (− cyt. aa3) | +IPTG (+ cyt. aa3) | −IPTG (− cyt. aa3) | |
| 168A (wild-type) | 0.95 | 1.0 | 1.1 | 1.1 |
| LUH15 (ΔctaCD) | 1.1 | 1.1 | n.d. | n.d. |
| LUW20 (ΔcydABCD) | 1.1 | 0 | 0.98 | 0.88 |
| LUW23 (ΔctaCD ΔcydABCD) | 1.2 | 0 | 1.1 | 0.98 |
a
Transformants were selected on plates containing chloramphenicol, with (+ cytochrome aa3) or without (− cytochrome aa3) 1 mM IPTG, incubated with (+O2) or without (−O2) oxygen. A value of 1.0 corresponds to 1,102 transformants. n.d., not done.