Skip to main content
. 2024 May 29;44(5):BSR20230667. doi: 10.1042/BSR20230667

Figure 6. Assessment of cell death parameters in GBM spheroids following TMZ treatment.

Figure 6

(A) Relative Luminescence (RLU) of Caspase 3/7 activity in U251MG WTE and TLS Polκ KO 3D tumor spheroids after treatments with TMZ and respective controls at Day 5. (B,C) Cell Population (%) with Positive Propidium Iodide (PI) staining fluorescence in 3D tumor spheroids from U251MG WTE (B) and TLS Polκ KO (C) cells after treatments with TMZ and respective controls at Day 3 and Day 5. (D,E) Photomicrographs of U251MG WTE (D) and TLS Polκ KO (E) tumor spheroids obtained after treatments with TMZ and respective controls at Day 0, Day 3, and Day 5. All images were obtained by the EVOS XL core microscope using RFP (PI) and DAPI (Hoechst) channels and with the 10× objective. The acquired images were analyzed and quantified by the software Fiji v. 3.1. Scale: 400 μm (white bar). All data were represented as means ± standard deviation (X ± SD) from four spheroids (n=4) per replicate and three independent biological experiments (n=3). *Values statistically different from the U251MG WTE cells at the point (date) (*P<0.05, **P<0.01, ***P0.001;,****P<0.0001; Two-way ANOVA followed by Bonferroni post-test). PC: Positive Control (Cisplatin 100 μM); SC: Solvent Control (1% DMSO); TMZ: Temozolomide.