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. 2024 May 30;15(5):380. doi: 10.1038/s41419-024-06760-0

Fig. 6. TBB ameliorates progeroid features and extends lifespan of Zmpste24-deficient mice.

Fig. 6

A Zmpste24+/+, Zmpste24−/−, and TBB-fed Zmpste24−/− mice at 4 months old; TBB (50 μM/L) or vehicle DMSO was dissolved in drinking water daily. B Survival and life span of TBB-fed Zmpste24−/− mice (n = 28) compared with those of the vehicle-fed controls (n = 25) using Kaplan–Meier analysis. A survival rate of 50% was observed at 18 and 24 weeks respectively in Zmpste24−/− and TBB-fed Zmpste24−/− mice. C Body weight curves of male, TBB-fed Zmpste24−/− mice (n = 11) were compared with vehicle controls (n = 9) or wild-type mice (n = 5). D Micro-CT analysis showing increased trabecular bone volume/tissue volume, trabecular number, and reduced trabecular separation in TBB-fed Zmpste24−/− mice (n = 5) compared with those of the vehicle-fed controls (n = 5). ***p < 0.001, ****p < 0.0001, two-tailed Student’s t -test. E Representative photos of SA-β-gal activity showing blue-stained senescent cells of kidney and spleen in TBB-fed Zmpste24−/− mice (n = 5) compared against those of vehicle-fed controls (n = 5). Scale bar, 50 μm. F Immunoblots showing P16, P21 and Bcl2 protein levels in different tissues isolated from male TBB-fed Zmpste24−/− and vehicle-fed control mice.