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. 2024 May 30;15:4605. doi: 10.1038/s41467-024-48220-5

Fig. 5. IRF3 increases the transcription of AIG1 in adipocytes.

Fig. 5

a Western blot of pAKT (S473) in WT SVF-derived adipocytes pre-treated with indicated conditioned medium plus insulin (100 nM) for 30 min. b Scheme showing the experimental paradigm used to identify IRF3 target genes by RNA-sequencing. Created with Biorender.com. c Volcano plot of RNA-seq dataset from (b). Genes that meet both significance and abundance are shown in black. Irf3 and Aig1 are marked in red. mRNA levels of Aig1 (n = 3) (d) and western blot (e) of AIG1 in WT and FI3KO, FI3OE SVF-derived adipocytes. mRNA levels of Aig1 in iWAT, eWAT, and BAT from WT, FI3KO (f) and FI3OE mice (g) (n = 5). h Western blot of AIG1 in iWAT, eWAT, and BAT of WT and FI3KO mice (n = 3). i Western blot of AIG1 in iWAT, eWAT, and BAT of WT and FI3OE mice (n = 3). Statistical significance was assessed by two-tailed Student’s t test. Data in all panels are expressed as mean ± SEM.