Figure 1.

Gating strategy of Gamma Delta T cells and different subsets. (A) Samples were initially gated on lymphocytes based on SSC-A/FSC-A gating. (B) Single cells were gated from lymphocytes using FSC-H/FSC-A. (C) T cells were gated from singlets as CD3⁺ cells. (D) Gamma delta T cells were gated from CD3⁺ as TCRγδ⁺. (E) Different subsets of Gamma delta T cells were characterized TCR Vδ1 and TCR Vδ2 markers as Vδ1⁺, Vδ2⁺ and Vδ1^-Vδ2^-.