FIG. 5.

Developmental expression of asgE in signaling mutants. The asgE::lacZ reporter gene fusion Ω5003 was introduced into mutants as described in Materials and Methods, and β-galactosidase-specific activity (defined as nanomoles of ONP produced minute⁻¹ milligram of protein⁻¹) was monitored at various times during development on TPM agar. Mean β-galactosidase-specific activities were determined from three independent experiments. Error bars represent the standard deviations of the mean. β-Galactosidase-specific activities for strain MS2021 (black squares) are compared to those of relA strain MS2036 (black triangles) (A); asgA strain MS2036 (black circles), asgB strain MS2037 (empty circles), and asgC strain MS2039 (empty triangles) (B); and csgA strain MS2041 (empty diamonds) (C).