TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| E. coli strain | ||
| DH5α | supE44 ΔlacU169 φ80ΔlacZM15 hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 26 |
| M. xanthus strains | ||
| DK101 | pilQ1 (wild-type development) | 13 |
| DK476 | pilQ1 asgA476 | 10 |
| DK480 | pliQ1 asgB480 | 10 |
| DK527 | pilQ1 relA | 12 |
| DK767 | pilQ1 asgC767 | 10 |
| DK5216 | pilQ1 csgA::Tn5132 (ΩLS205) | 33 |
| MS2020 | pilQ1 orf2::pBMG3 (Ω5002) | 9 |
| MS2021 | pilQ1 asgE::pREG-JP2B (Ω5003) | 9 |
| MS2036 | pilQ1 relA asgE::pREG-JP2B (Ω5003) | This study |
| MS2037 | pilQ1 asgB480 asgE::pREG-JP2B (Ω5003) | This study |
| MS2038 | pilQ1 asgA476 asgE::pREG-JP2B (Ω5003) | This study |
| MS2039 | pilQ1 asgC767 asgE::pREG-JP2B (Ω5003) | This study |
| MS2040 | pilQ1 orf2::pBMG4 (Ω5005) | This study |
| MS2041 | pilQ1 csgA::Tn5132 (ΩLS205) | This study |
| asgE::pREG-JP2B (Ω5003) | ||
| Plasmids | ||
| pBGS18 | Kanr | 34 |
| pBluescript II SK | Ampr | Stratagene |
| pREG1727 | Ampr Kanr | 6 |
| pBMG3 | Kanr, pBGS18 containing 0.5 kb of orf2 on a XbaI-BamHI fragment | 9 |
| pBMG4 | Ampr Kanr, pREG1727 containing 1.0 kb of orf2 and 1.5 kb of upstream DNA on an XhoI-BamHI fragment | This study |
| pELF3 | Kanr, pBGS18 containing orf2, asgE, and 0.4 kb of downstream DNA on a BamHI-PstI fragment | 9 |
| pREG-JP2B | Ampr Kanr, pREG1727 containing 1.0 kb of asgE on a BamHI-HindIII fragment | 9 |