Figure 1.

(A) Hierarchical clustering of the differentially expressed miRNAs in ESCC cell culture media. Hypoxia affected miRNA expression in KYSE-960 and T.Tn cell culture media. In the KYSE-960 cell line, 33 miRNAs were downregulated and 12 were upregulated. In the T.Tn cell line, 33 miRNAs were downregulated and nine were upregulated. The color intensity represents the magnitude of expression difference; red indicates miRNAs that were 2-fold upregulated and green indicates miRNAs that were 2-fold downregulated in normoxic cells compared with hypoxic cells. The expression values are log-transformed. (B) Exosomal miR-185 expression, as calculated by detection above background algorithm and normalized by robust multichip analysis, was downregulated in both KYSE-960 and T.Tn cell line culture media (KYSE-960 fold-change: 0.32; T.Tn fold-change: 0.22). miRNA/miR, microRNA.