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(A) Cell apoptosis was evaluated following Annexin V-FITC and propidium iodide staining by flow cytometry. (B) Reverse transcription-quantitative PCR was used to the detect the mRNA expression levels of BCL-2 in KYSE-960 and T.Tn cell lines. β-actin was used as the housekeeping gene. (C) Percentage of cells in each stage of the cell cycle. **P<0.01, ***P<0.001 vs. negative control. miR, microRNA.
