FIG. 7.

Western blot analysis of cytoplasmic levels of SspA. Bacterial strains deficient for secretion were grown overnight, back diluted, and grown to exponential growth phase. Samples from ΔprgH (TK164) and ΔprgH ΔinvB (PB509) strains were collected, resolved using SDS-PAGE, and analyzed by Western blotting using antibodies specific for SspA. The amount of SspA present in these strains was quantitated using a STORM840 Phosphoimaging System and by analyzing the data with Imagequant V1.2. Percent SspA levels are presented with standard deviations of triplicate samples. These experiments were repeated multiple times.