Skip to main content
. Author manuscript; available in PMC: 2024 May 31.
Published in final edited form as: J Autoimmun. 2023 Jul 1;139:103084. doi: 10.1016/j.jaut.2023.103084

Figure 2. X-linked gene expression is not affected by lupus-like disease in in vitro activated CD23+ B cells from female MRL/lpr mice.

Figure 2.

A. Mean fold-change ± SD of Xist expression in CD23+ B cells at 24-hours post-activation with CpG from female MRL/lpr mice (n=14) relative to female B6 mice (n=9) and from female NZM2328 mice (n=19) relative to female B6 (n=10). B. Schematic depicting the approach used to infer gene expression from the Xi. The locations of the X-linked genes used for qPCR analyses in panel C is shown. C. Rpl13a-normalized expression for seven X-linked genes with immune functions and that are distributed broadly over the X chromosome in activated CD23+ B cells from MRL/lpr mice, determined via qPCR. The mean fold-change (± SD) for a given gene was calculated based its expression in 24-hour-CpG-activated CD23+ splenic B cells from 8–31 week-old female MRL/lpr mice (n=14–16) and age-matched male MRL/lpr mice (n=15–16) relative to female B6 mice (n=9–10) using the 2−ΔΔCt approach. ns = not statistically significant.