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. Author manuscript; available in PMC: 2024 May 31.
Published in final edited form as: J Autoimmun. 2023 Jul 1;139:103084. doi: 10.1016/j.jaut.2023.103084

Figure 4. Lupus-like disease does not alter X-linked gene expression in in vitro activated CD3+ T cells from female MRL/lpr mice.

Figure 4.

A. Mean fold-change ± SD of Xist expression at 48-hours post-activation with anti-CD3/anti-CD28 relative to 0h (non-activated) for CD3+ T cells from female MRL/lpr (n=14) and NZM2328 mice (n=18), relative to female B6 mice (n=10), stratified by mouse age based on data in Figure 3E. B. Rpl13a-normalized expression for five X-linked genes with relevant immune functions (Cxcr3, Ogt, Cd40l, Irak1, Msn) in 48-hr activated T cells from MRL/lpr mice, determined via qPCR. The mean fold-change (± SD) for a given gene was calculated based on its expression in 48-hour-anti-CD3/CD28-activated CD3+ T cells from 18–31 week-old female MRL/lpr mice (n=9–10) and age-matched male MRL/lpr mice (n=8–9) relative to female B6 mice (n=6). 18–31-week-old mice were specifically selected for this analysis as this age range exhibited impaired Xist RNA localization (Figure 3E). ns = not statistically significant.