FIG. 3.

Identification of the reaction product from CHMS catalyzed by LigC. (A) Gas chromatogram of the TMS derivative of the reaction product from CHMS catalyzed by purified LigC. CHMS (100 μM) was incubated with 3 μg of purified LigC for 2 min in the presence of 200 μM NADP⁺. The reaction product was extracted by ethylacetate and trimethylsilylated (TMS). (B) Mass spectrum of the TMS derivative of the product with a retention time of 27.8 min shown in panel A. (C) Mass spectrum of the authentic TMS-PDC.