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. 2000 Dec;182(23):6651–6658. doi: 10.1128/jb.182.23.6651-6658.2000

TABLE 2.

Purification of CHMS dehydrogenase from E. coli BL21(DE3) harboring pSM21

Fraction Total protein (mg) Total activity (U) Sp act (U/mg) Yield (%) Fold
Crude extract 40.7 1,040 25.6 100 1
PI 3.23 394 122 38 4.8
HQ 0.70 182 260 18 10
PE 0.30 108 360 10 14