TABLE 3.
Kinetic constants of the CHMS dehydrogenases
| Strain | Cofactor (μM) | CHMS (μM) | Km (μM)a | Vmax (U/mg) |
|---|---|---|---|---|
| SYK-6 | NAD+ | 150 | 252 ± 3.9 (NAD+) | 449 ± 3.9 |
| NADP+ | 150 | 24.6 ± 1.5 (NADP+) | 363 ± 1.4 | |
| NAD+ (200) | 20.6 ± 1.0 (CHMS) | 175 ± 10 | ||
| NADP+ (200) | 26.0 ± 0.5 (CHMS) | 383 ± 3.8 | ||
| P. ochraceaeb | NAD+ | 158 | 284 (NAD+) | 470 |
| NADP+ | 158 | 17.5 (NADP+) | 470 | |
| NAD+ (110) | 56 (CHMS) | 167 | ||
| NADP+ (110) | 20 (CHMS) | 450 |
a
The Kms indicated are the values for the compounds shown in parentheses.
b
The results for the P. ochraceae enzyme have been reported in a previous study (17).