Skip to main content
. Author manuscript; available in PMC: 2024 May 31.
Published in final edited form as: Hepatology. 2023 Jan 3;78(3):896–910. doi: 10.1097/HEP.0000000000000019

FIGURE 1.

FIGURE 1

Chronic alcohol consumption leads to a reduction of intestinal basic leucine zipper transcription factor ATF-like 3 (Batf3)-dependent conventional type 1 dendritic cells (cDC1s) in mice. C57BL/6J wild-type (WT) mice were fed Lieber-DeCarli liquid diets containing alcohol (alcohol-fed, AF) or isocaloric dextran (pair-fed, PF) for 8 weeks plus a single binge (4 g/kg). (A) Gating strategy and representative dot plot of singlet 7-AAD−CD45+CD64−CD11c+MHCII+ total cDCs (n = 10). (B) Heatmap plot of the proteomic changes of the purified ileal cDCs (n = 7–8). (C) Dot plot of intestinal cDC1s (n = 10). (D) Analysis of mRNA levels of ileal Batf3, Clec9a, and Xcr1 (n = 6). (E) IL-12 levels quantified by ELISA (n = 6). (F) Th1 (CD3+CD4+IFN-γ+) cells (n = 10), CD3+CD8+IFN-γ+ cells (n = 7), and protein interferon gamma (IFN-γ) levels (n = 6) in mouse ileum. Data are presented as mean ± SD. Statistical comparisons were made using Student t test; **P < 0.01 versus PF mice.