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. Author manuscript; available in PMC: 2024 May 31.
Published in final edited form as: Hepatology. 2023 Jan 3;78(3):896–910. doi: 10.1097/HEP.0000000000000019

FIGURE 2.

FIGURE 2

Lack of basic leucine zipper transcription factor ATF-like 3 (Batf3)-dependent conventional type 1 dendritic cells (cDC1s) exacerbates alcohol-induced gut barrier disruption and pathogen-associated molecular patterns translocation into the liver. C57BL/6J wild-type (WT) mice and Batf3−/− mice were fed Lieber-DeCarli liquid diets containing alcohol or isocaloric dextran for 8 weeks plus a single binge (4 g/kg). (A) Dot plot of ileal cDC1s (n = 10). (B) Ileal Th1 cells proportion (n = 7). (C) Ileal interferon gamma (IFN-γ) protein levels (n = 7) and the mRNA levels of ileal Ip-10 (n = 6). (D) The mRNA levels of ileal tight junctions (n = 6) and IF staining of ileal ZO-1 and OCCLUDIN (n = 6). (E) Plasma and hepatic LPS levels (n = 7). (F) Ileal Tnf-α mRNA levels (n = 6). Data are presented as mean ± SD. *P < 0.05, **P < 0.01 versus WT/PF mice; #P < 0.05, ##P < 0.01 versus WT/AF mice. AF, alcohol-fed; PF, pair-fed; LPS, lipopolysaccharide.