Skip to main content
. Author manuscript; available in PMC: 2024 May 31.
Published in final edited form as: Hepatology. 2023 Jan 3;78(3):896–910. doi: 10.1097/HEP.0000000000000019

FIGURE 4.

FIGURE 4

Adoptive transfer of conventional type 1 DCs (cDC1s) recovered alcohol-induced Akkermansia muciniphila abundance reduction and liver injury in mice. Alcohol-fed C57BL/6J wild-type (WT) mice were administrated with or without cDC1s adoptive transfer. (A) cDC1 were specific and efficiently generated. IL-12 protein levels in isolated DCs and ileal interferon gamma (IFN-γ) protein levels (n = 7). (B) The mRNA levels of ileal Occludin and Zo-1 (n = 6). (C) Plasma and hepatic LPS levels (n = 6). (D) Representative dot plot and frequency of neutrophils in the liver (n = 6). The mRNA levels of hepatic Cxcl1 and Lcn2 (n = 6). (E) Liver hematoxylin and eosin (H&E) staining and hepatic TG contents (n = 6). Scale bars: 50 μm. Asterisks: lipid droplets. (F) Plasma alanine aminotransferase (ALT) levels (n = 7). Data are presented as mean ± SD. Statistical comparisons were made using Student t test. *P < 0.05, **P < 0.01 versus alcohol-fed (AF) mice. LPS indicates lipopolysaccharide; TG, triglyceride.