Skip to main content
. Author manuscript; available in PMC: 2024 May 31.
Published in final edited form as: Hepatology. 2023 Jan 3;78(3):896–910. doi: 10.1097/HEP.0000000000000019

FIGURE 5.

FIGURE 5

Lack of conventional type 1 DCs (cDC1) in gut exacerbated alcohol-reduced Akkermansia muciniphila abundance. (A–C) C57BL/6J wild-type (WT) mice and Batf3−/− mice were fed Lieber-DeCarli liquid diets containing alcohol or isocaloric dextran for 8 weeks plus a single binge (4 g/kg). (A) PCoA plot showing dissimilarity in bacterial community structures based on J-class and Bray-Curtis distances, respectively (n = 6). Barplot showing the cecal bacterial compositions at the family level (left) and genus (right) level, respectively (n = 6). (B) Relative ileal A. muciniphila abundance (n = 6). (C) The mRNA levels of ileal Defa5 (n = 6). (D–E) Alcohol-fed C57BL/6J WT mice were administrated with or without cDC1s adoptive transfer. (D) Relative ileal A. muciniphila abundance (n = 6). (E) The mRNA levels of ileal Defa5 (n = 6). (F) Alcohol-fed C57BL/6J WT mice and Batf3−/− mice were administrated with or without interferon gamma (IFN-γ), respectively. (F) Relative A. muciniphila abundance and the mRNA levels of ileal antimicrobial peptides (AMPs) (n = 6). In panels A–C, data are presented as mean ± SD. **P < 0.01 versus WT/pair-fed (PF) mice; #P < 0.05, ##P < 0.01 versus WT/AF mice. In panels D and E, data are presented as mean ± SD. **P < 0.01 versus alcohol-fed (AF) mice. In panel F, data are presented as mean ± SD. *P < 0.05, **P < 0.01 versus WT/AF mice; ##P < 0.01 versus knockout (KO)/AF mice. PCoA, principal coordinates analysis.