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. Author manuscript; available in PMC: 2024 May 31.
Published in final edited form as: Hepatology. 2023 Jan 3;78(3):896–910. doi: 10.1097/HEP.0000000000000019

FIGURE 6.

FIGURE 6

Akkermansia muciniphila supplementation ameliorated alcohol-induced tight junction disruption and pathogen-associated molecular patterns (PAMPs) translocation in both wild-type (WT) mice and Batf3−/− mice. Alcohol-fed C57BL/6J WT mice and Batf3−/− mice were administrated with or without A. muciniphila, respectively. (A) The mRNA levels of ileal Zo-1, Occludin, and Claudin1 (n = 6) and the IF staining of ileal ZO-1 and OCCLUDIN. Positive signals of ZO-1 and OCCLUDIN were stained in red, respectively, while nuclei were stained by DAPI in blue. Scale bar, 50 μm. (B) Plasma and hepatic LPS levels (n = 5). (C) The mRNA levels and protein levels of hepatic lipocalin-2 (LCN2) (n = 6). Positive signals of LCN2 were stained in green, while nuclei were stained by DAPI in blue. Scale bar, 50 μm. (D) Hepatic protein levels of p-signal transducer and activator of transcription 3 (STAT3) and STAT3 (n = 3). (E) The numbers of ileal CD103+CD11b− cDC1s (n = 10). (F) Relative ileal Ifn-γ mRNA levels (n = 6). Data are presented as mean ± SD. *P < 0.05, **P < 0.01 versus WT/alcohol-fed (AF) mice; #P < 0.05, ##P < 0.01 versus knockout (KO)/AF. DAPI, 4′,6-diamidino-2-phenylindole IF, immunofluorescence; LPS, lipopolysaccharide.