TABLE 4.
Effect of plasmid-borne epsR on expression from wild-type and mutant eps promoters in R. solanacearum
| Straina |
eps expression (Miller units) in presence of:
|
|
|---|---|---|
| pLAFR3 | pKL4 | |
| AW19A (epsB::lacZ) | 1,980 | 253 |
| AW (pPSZ12) [−143 to +23] | 710 | 60 |
| AW (pPSZ19) [−101 to +23] | 568 | 57 |
| AW (pPSZ22) [−44 to +23] | 34 | 31 |
| AW (pEPSM9) [−143 to +23; −70 G to A] | 55 | 44 |
| AW (pEPSM3) [−143 to +23; −74 G to A] | 51 | 37 |
a
R. solanacearum strains contained either the pLAFR3 vector or pKL4 (pLAFR3 carrying epsR). AW19A is a wild-type strain with a genomic epsB::lacZ reporter. AW strains harbored the indicated Peps::lacZ reporter plasmids (see Fig. 1); numbers in brackets indicate region of Peps fused to lacZ and mutation, if any. eps expression was measured as in Fig. 1.